EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.1.19 | gene bedD encoding cis-benzene dihydrodiol dehydrogenase on the natural plasmid pHMT112, expressed in Escherichia coli | Pseudomonas putida |
1.3.1.19 | sequenze similarity to glycerol dehydrogenase of Escherichia coli | Pseudomonas putida |
1.3.1.25 | - |
Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.1.19 | 39000 | - |
4 * 39000, SDS-PAGE | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.19 | cis-1,2-dihydrobenzene-1,2-diol + NAD+ | Pseudomonas putida | - |
catechol + NADH | - |
? | |
1.3.1.19 | cis-1,2-dihydrobenzene-1,2-diol + NAD+ | Pseudomonas putida ML2 | - |
catechol + NADH | - |
? | |
1.3.1.25 | (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ | Pseudomonas putida | enzyme is involved in the metabolism of benzene. A bedD mutant in which the gene is disrupted by a kanamycin resistance cassette is unable to utilize benzene for growth | catechol + CO2 + NADH | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.19 | Pseudomonas putida | - |
- |
- |
1.3.1.19 | Pseudomonas putida ML2 | - |
- |
- |
1.3.1.25 | Pseudomonas putida | - |
- |
- |
1.3.1.25 | Pseudomonas putida ML2 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.1.19 | dye-affinity chromatography and NAD+ agarose chromatography | Pseudomonas putida |
1.3.1.25 | - |
Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.1.25 | 89.1 | - |
- |
Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.19 | 1,2-propanediol + NAD+ | - |
Pseudomonas putida | propen-1,2-diol + NADH | - |
? | |
1.3.1.19 | 1,2-propanediol + NAD+ | - |
Pseudomonas putida ML2 | propen-1,2-diol + NADH | - |
? | |
1.3.1.19 | cis-1,2-cyclohexanediol + NAD+ | - |
Pseudomonas putida | cyclohex-1-ene-1,2-diol + NADH | - |
? | |
1.3.1.19 | cis-1,2-cyclohexanediol + NAD+ | - |
Pseudomonas putida ML2 | cyclohex-1-ene-1,2-diol + NADH | - |
? | |
1.3.1.19 | cis-1,2-dihydrobenzene-1,2-diol + NAD+ | - |
Pseudomonas putida | catechol + NADH | - |
? | |
1.3.1.19 | cis-1,2-dihydrobenzene-1,2-diol + NAD+ | - |
Pseudomonas putida ML2 | catechol + NADH | - |
? | |
1.3.1.25 | (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ | enzyme is involved in the metabolism of benzene. A bedD mutant in which the gene is disrupted by a kanamycin resistance cassette is unable to utilize benzene for growth | Pseudomonas putida | catechol + CO2 + NADH | - |
? | |
1.3.1.25 | 1,2-propanediol + NAD+ | at 189% of the activity with cis-benzene dihydrodiol | Pseudomonas putida | ? | - |
? | |
1.3.1.25 | 1,2-propanediol + NAD+ | at 189% of the activity with cis-benzene dihydrodiol | Pseudomonas putida ML2 | ? | - |
? | |
1.3.1.25 | cis-1,2-cyclohexanediol + NAD+ | at 95% of the activity with cis-benzene dihydrodiol | Pseudomonas putida | ? | - |
? | |
1.3.1.25 | cis-benzene dihydrodiol + NAD+ | - |
Pseudomonas putida | dihydroxybenzene + NADH | - |
? | |
1.3.1.25 | glycerol + NAD+ | at 18% of the activity with cis-benzene dihydrodiol | Pseudomonas putida | ? | - |
? | |
1.3.1.25 | glycerol + NAD+ | at 18% of the activity with cis-benzene dihydrodiol | Pseudomonas putida ML2 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.1.19 | tetramer | 4 * 39000, SDS-PAGE | Pseudomonas putida |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.19 | cis-benzene dihydrodiol dehydrogenase | - |
Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.19 | NAD+ | - |
Pseudomonas putida |